Figure 5.

Annonaceous acetogenins affect on AKT/NF-κB Signal Pathway Molecules in BEL-7402/5-FU and HepG2/ADM cells. A. The total cell lysate was separated by SDS-PAGE gel followed by Western blot with anti-p-Akt antibody, anti-total-Akt antibody. The experiment shown is epresentative of three independent experiments with similar results; B. Cells were transfected with pNF-κB-luc and the transcriptional activation was detected by luciferase reporter assay. Data were presented as the mean ± SD and were representative of an average of three independent experiments per concentration. Significant differences from untreated control are indicated by P<0.05 (*) and P<0.01 (**).