Figure 1.

Analysis of Prdx1 expression in human hilar cholangiocarcinoma and adjacent non-cancerous specimens. (A) Normal tissues showed negative staining of Prdx1; (B) Strong positive staining of Prdx1 in the cytoplasm of cancer cells; (C) Weak staining of Prdx1 in cancer cells; (D) Negative staining of Prdx1 in cancer cells; (E) Western blot analysis of three paired cancer specimens (T) and adjacent non-cancerous specimens (N); (F) Relative amounts of protein in these specimens vs β-actin from (E) analyzed by Image J. Original magnification of (A-D) 40 ×; Original magnification of small pictures in (A-D) 200 ×.