Figure 5.

Silencing of Prdx1 by specific Prdx1-shRNA inhibits cancer cell proliferation and colony formation. QBC939 cells were transfected with Prdx1-shRNA for 48 hours and immunofluorescence assay (A) and Western blotting (B) was used to detect the expression status of Prdx1; (C) QBC cells transfected with Prdx1-shRNA and CCK8 assay was performed at 48 hours; *P<0.05, compared with control. (D, E) QBC cells transfected with Prdx1-shRNA were grown in 6-well plates and incubated with 10% FBS for 2 weeks. The numbers of the cell colonies (>50 cells) were obtained and calculated as: colonies/500 × 100. *P<0.05, compared with control.