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. 2015 Sep 1;8(9):9889–9900.

Figure 3.

Figure 3

72 hours after transfected with miR-96 mimics, inhibitor and NC (100 nmol/l), the relative expression of FOXO1and Bim in PTC cell lines K1 and TPC1. A. The relative expression of FOXO1 mRNA in K1and TPC1 cells, which was analyzed by RT-qPCR. The graph represents the 2-ΔΔCt values ± SEM, **P < 0.01; ***P < 0.001 (two tailed t test). B. The relative expression of FOXO1 and Bim protein in K1 and TPC1 cells, which analyzed by western blot analysis. β-actin was used as a loading control. C. The graph shows the mean ±SEM of FOXO1 and Bim protein levels relative to their respective loading controls. Quantitative analysis was conducted by measuring the IDV of protein bands. *P < 0.05; **P < 0.01 (two tailed t test). NC; negative control; SEM, standard error of the mean; IDV, integrated density value.