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. 2015 Nov 9;5:16289. doi: 10.1038/srep16289

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Copyright © 2015, Macmillan Publishers Limited

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(A) Western blot analysis of Δldaap24 ectopically co- expressing Δ18LdAAP24 or Δ44LdAAP24 (39 kDa and 37 kDa, respectively) and the full length N terminus (10 kDa). (B) Initial transport rate (3 minutes) of 1 mM ³H L-alanine was assayed in LdAAP24-null mutants ectopically expressing Δ18LdAAP24/Δ44LdAAP24 (−N’, dark grey) and LdAAP24-null mutants ectopically co-expressing Δ18LdAAP24/Δ44LdAAP24 and the full length N terminus (+N’, light grey). Transport was determined at pH 7 and 30 °C. Values represent the mean of at least three independent repeats ± SD. Statistically significant differences (p ≤ 0.01) are marked with (**).