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. 2015 Nov 9;5:16289. doi: 10.1038/srep16289

Figure 5. The soluble N-terminus cannot recover proline transport.

Figure 5

(A) Western blot analysis of ΔLdAAP24 ectopically co- expressing Δ54LdAAP24 (36 kDa) and the full length N terminus (10 kDa). (B) Initial transport rate (3 minutes) of 1 mM 3H L-proline or 1 mM 3H L-alanine were assayed in LdAAP24-null mutants ectopically expressing Δ54LdAAP24 (−N’, dark grey) and LdAAP24-null mutants ectopically co-expressing Δ54LdAAP24 and the full length N terminus (+N’, light grey). Transport was determined at pH 7 and 30 °C. Values represent the mean of at least three independent repeats ± SD.