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. 2015 Nov 9;5:15701. doi: 10.1038/srep15701

Figure 2. The relationship between IL-6 and autophagy.

Figure 2

(a) The expression of IL-6R mRNA relative to β-actin was quantified by RT-PCR under starvation incubated with or without IL-6 at the indicated concentration for 2 h. (b) The LC3 lipidation state was assayed by immunoblotting under starvation conditions and incubation with or without IL-6 at the indicated concentration for 2 h. (c) The endogenous LC3 puncta were observed by immunofluorescence method under starvation conditions with or without IL-6 (30 ng/mL) or 3-MA (10 mM) or baf A1 (100 nM) for 2 h. (d) Endogenous LC3 puncta per cell were quantified. Scale bars represented 5 μm.