Figure 2. The behaviour tests in P30 LKB1^Atoh1 CKO and wild-type mice.

(A) Footprint assay in gait test in wild-type mice and LKB1^Atoh1 CKO mice. The stride width (cm) of hindpaw footprint was significantly wider in the mutant mice compared with wild-type mice (P = 5 × 10 ⁻⁷). (B) Rotarod tests were performed on wild-type and LKB1^Atoh1 CKO mice for 5 consecutive days using a rotarod treadmill. The duration time for the mice to stay on the rotary rod at 10 rpm was counted and compared. The mutant mice exhibited impaired motor coordination with a shorter duration time compared to the wild-type mice (P = 0.0036; 0.0028; 0.00014; 0.0074; 0.0072). (C) Tightrope suspension time (P = 0.042) and crossing (P = 0.049) were measured in three trials in tightrope test. Transformed scores (P = 8.2 × 10 ⁻⁴) were calculated. Results showed that transformed score was significantly lower in the mutant mice than those of wild-type mice. The error bars indicate the SEM. *P < 0.05; **P < 0.01; ***P < 0.001 compared to the WT by Student’s t-test; n = 6 animals for each group.