Figure 7. UBC overproduction in the LKB1^Atoh1 CKO mice.

(A) Cerebellar sections were stained with an anti-Tbr2 antibody, a marker of UBCs, and imaged in folia IX and X. The P3.5 and P8 LKB1^Atoh1 CKO cerebellum showed an obvious increase in the number of Tbr2⁺ cells, compared to the controls. Scale Bars: 100 μm. (B) The P8 LKB1^Atoh1 CKO cerebellum showed a large number of Tbr2⁺ cells in folia VI and VII, while only a few Tbr2⁺ cells were observed in the wild-type cerebellum. (C) Western blot analysis of the Tbr2 protein in the wild-type and mutant cerebellum. The results showed that the level of the Tbr2 protein was significantly increased in the P3.5 and P8 LKB1^Atoh1 CKO mice (CKO) compared to the controls (WT). (C’) Quantification of the Western blot results for the Tbr2 protein in the wild-type (WT) and LKB1^Atoh1 CKO cerebellum (CKO). The data were normalized against the results from the wild-type (P_P3.5 = 2.4 × 10 ⁻³; P_P8 = 6 × 10 ⁻⁵). The error bars indicate the SEM. *P < 0.05; **P < 0.01; ***P < 0.001 compared to the WT by Student’s t-test; n = 4 animals for each group.