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. 2015 Nov 9;5:16492. doi: 10.1038/srep16492

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(A) IHC staining for α-SMA in HCC mice. The arrows indicate α-SMA-positive myofibroblasts of liver tissues in HCC mice. Scale bars: 50 μm. (B) Effects of conditioned media (CM) from TGF-β activated LX-2 cells on the proliferation of SK-HEP1, SNU354, and HepG2 cells. Cells were treated with CM for 72 h. HI, heat-inactivated; CHX, cycloheximide (10 μg/ml). (C) Effects of CM on the migration of SK-HEP1 and HepG2 cells. Cells were treated with CM for 30 h. Scale bars: 200 μm. (D) Effects of CM the anchorage-independent growth of SK-HEP1 cells. Cells were treated with CM for 24 h. After treatment, the cells were counted and placed in soft agar for colony assay to determine cell survival. ^***p < 0.001 vs. untreated control and ^###p < 0.001 vs. CM-treated control.