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. Author manuscript; available in PMC: 2015 Nov 9.
Published in final edited form as: Am J Physiol Cell Physiol. 2001 Dec;281(6):C1825–C1836. doi: 10.1152/ajpcell.2001.281.6.C1825

Fig. 10.

Fig. 10

Nuclear run-on transcription assay. ARPE-19 cells were incubated for 24 h with or without 1 mM SIN-1. Nuclei were isolated, and nascent RNA was labeled with [α-32P]UTP. This radiolabeled RNA was then hybridized to membranes with cross-linked human taurine transporter cDNA. Membranes were hybridized overnight at 68°C and exposed to X-ray film.