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Immunodetection of nitrotyrosine in ARPE-19 cells exposed to SIN-1. ARPE-19 cells were grown to confluency on laminin-coated chamber slides and then exposed to 1 mM SIN-1 for 2 h. Cells were processed for immunohistochemistry using a primary antibody against nitrotyrosine followed by an FITC-labeled secondary antibody. A: SIN-1 treated cells. B: control cells. Magnification ×600.
