Fig. 8.

Effects of cycloheximide and actinomycin D on SIN-1-induced increase in taurine transporter activity. Confluent ARPE-19 cells were pretreated with cycloheximide (100 μg/ml) or actinomycin D (10 μg/ml) for 2 h and then incubated with these compounds in the presence or absence of 1 mM SIN-1. Uptake of [³H]taurine (80 nM) was then measured for 15 min. Values are means ± SE for 3 determinations from 3 independent experiments.