Table 2. Specificity of SIN-1-induced stimulation of taurine uptake.
Uptake, pmol·mg protein−1·15 min−1 |
||
---|---|---|
Substrate | Control | SIN-1 |
Taurine (80 nM) | 2.58 ± 0.088(100) | 10.65 ± 0.053(413) |
Glutamine (50 nM) | 5.64 ± 0.388(100) | 6.07 ± 0.269(108) |
Glutamate (100 nM) | 13.75 ± 0.152(100) | 13.93 ± 0.583(101) |
Carnitine (30 nM) | 0.41 ± 0.024(100) | 0.37 ± 0.032(90) |
Leucine (30 nM) | 1.75 ± 0.085(100) | 2.08 ± 0.006(118) |
Alanine (30 nM) | 2.22 ± 0.059(100) | 2.44 ± 0.086(110) |
Arginine (30 nM) | 2.30 ± 0.101(100) | 2.08 ± 0.090(90.4) |
Values are means ± SE; values in parentheses are percentages of respective control uptake. ARPE-19 cells were treated with or without 1 mM SIN-1 for 24 h, and then uptake of [3H]taurine and other radiolabeled amino acids was measured for 15 min.