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. 2015 Sep 3;106(10):1303–1312. doi: 10.1111/cas.12755

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© 2015 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Overexpression of DDX5 stimulates non-small-cell lung cancer cell proliferation. (a) Squamous carcinoma H520 cells and adenocarcinoma A549 cells were stably infected with DDX5 or vector. The expression of DDX5 was analyzed by Western blot. (b) Cell growth curves as determined by MTT assay. (c) Percentage of EdU-positive cells determined by EdU incorporation assay (lower panel) and representative images of EdU staining (upper panel). (d) Effects of upregulated DDX5 on the clonogenic ability of H520 and A549 cells. Representative images are shown in the upper panel and the number of colonies illustrated in the lower panel. *P < 0.05.