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. 2015 Sep 3;106(10):1303–1312. doi: 10.1111/cas.12755

Figure 7.

Figure 7

Suppression of β-catenin abrogates DDX5-induced cyclin D1 and c-Myc expression and cell proliferation. (a) Real-time PCR analysis of the expression of cyclin D1 and c-Myc. DDX5-infected H520 and A549 cells were subjected to transfection of siRNA targeting β-catenin (siBcat) or control siRNA (siCon) for 48 h. (b) Western blot analysis of the expression of DDX5, β-catenin, cyclin D1, and c-Myc. (c) Indicated cells were transfected with siBcat or siCon plus pGL3-cyclinD1 (firefly) and pRL-TK (Renilla) for 48 h and subjected to dual luciferase reporter assays. Relative cyclinD1-Luc activity was normalized by Renilla luciferase activity. (d) Indicated cells were transfected with siBcat or siCon plus TOPflash (TOP) or FOPflash (FOP) and pRL-TK (Renilla) plasmids for 48 h and subjected to dual luciferase reporter assays. Reporter activity was normalized by Renilla luciferase activity. (e) EdU incorporation assay revealed that inhibition of β-catenin abrogated DDX5-induced cell proliferation. *P < 0.05. NS, not significant.