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. 2015 Sep 30;106(10):1377–1384. doi: 10.1111/cas.12749

Figure 2.

Figure 2

Epithelial to mesenchymal transition in afatinib-resistant cell lines. (a) HCC827-AR1, -ACR, -AR4, HCC4006-AR1, and -AR2 displayed downregulation of E-cadherin and upregulation of vimentin. (b) Immunofluorescence cytochemistry of E-cadherin and vimentin on HCC827 and its several sublines are shown. HCC827 displayed epithelial phenotype although HCC827-AR1 and ACR displayed mesenchymal phenotype. Scale bars, 10 μm.