Figure 1.

Transgene-free reprogramming of SH-IN cells. (a) Schematic outlining the transgene-free reprogramming of SH-IN cells using a SeV vector. SH-IN cells were infected with the SeV vector encoding the transcription factors OCT4, SOX2, KLF4, and c-MYC. (b) Total RNA was extracted from cells at 6 days to 4 months post-transduction and analyzed by semi-quantitative RT-PCR to verify transgene elimination. (c) Typical morphology of parental SH-IN and foreskin fibroblast BJ cells (left panels). Formation of induced pluripotent stem cells (iPSCs)-like colonies after transduction (right panels). SH-IN 4F cells stained positive for alkaline phosphatase (AP, bottom row). Scale bar: 100 μm.