Figure 2.

SH-IN 4F cells express high levels of pluripotency-associated genes. (a) SH-IN 4F cells (clone 2) expressed undifferentiated embryonic stem cell (ESC) markers and surface antigens (NANOG, OCT4, SOX2, SSEA-4, TRA-1-60, and TRA-1-81) as determined by immunocytochemical analysis. Nuclei were stained with DAPI (blue). Results are representative of three independent experiments. Scale bar: 75 μm. (b) Epigenetic modification of pluripotency-related genes was examined by bisulfite genomic sequencing. (c) Reprogramming of SH-IN cells reduces NANOG promoter methylation. BJ and 201B7-iPSC lines are included as negative and positive controls, respectively. Values above each column indicate the CpG position examined from the translation initiation start codon. Each horizontal row of circles indicates the methylation status of CpG dinucleotides in one individual sequencing reaction of a bacterial clone. White circles indicate unmethylated CpGs and black circles indicate methylated CpGs. The proportion (%) of unmethylated CpGs is indicated below each cell line. Results are representatives of two independent experiments.