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. 2015 Sep 25;106(10):1438–1447. doi: 10.1111/cas.12756

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© 2015 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Distribution of doxorubicin fluorescence intensity in relation to blood vessels in EMT6- and MCF7-derived tumors. Mice were given either 25 mg/kg doxorubicin alone ((Dox; 10 min) (a, d) or 200 mg/kg lansoprazole (2 h before doxorubicin) and doxorubicin (Dox+Lanso) (b, e) prior to tumor excision. Tumors were imaged and quantified using a customized algorithm to show the mean absolute (background-subtracted) values of doxorubicin fluorescence intensity (blue) as a function of distance to the nearest functional blood vessel (green) (c, f). Scale bar = 100 μm.