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. 2015 Sep 1;14(11):2887–2900. doi: 10.1074/mcp.M115.050484

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 1. — Tamoxifen-resistant MCF7 cell line exhibits resistance to tamoxifen and increase in global phosphotyrosine levels. A, Inhibitory effect of different concentrations of tamoxifen on the proliferation of MCF7 tamoxifen-resistant (MCF7-TamR) cell line and MCF7 sensitive control (MCF7-CTRL) cell line. Cell proliferation was assessed using MTT assay after 7 days of treatment. Quantification was done relative to vehicle-treated MCF7-CTRL. Student's t test was performed for statistical analysis. B, Tumor size of xenograft on mice grown with either MCF7-TamR or MCF7-CTRL cell lines with estrogen (E2) supplementation and treated with or without tamoxifen (TAM). Two-way Anova test was performed for statistical analysis. C, Immunoblot of tyrosine phosphorylated proteins using the anti-phosphotyrosine antibody (pTyr) on the cell lysates or after immunoprecipitation (IP) with pTyr antibody. β-actin, heavy (H) chain and light (L) chain serve as loading control.