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. 2015 Sep 1;14(11):2887–2900. doi: 10.1074/mcp.M115.050484

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 2. — Workflow of phosphoproteomic profiling of tamoxifen-resistant and sensitive cell line. MCF7 tamoxifen-resistant (MCF7-TamR) cell line was labeled with light amino acids and MCF7 tamoxifen-sensitive control (MCF7-CTRL) cell line was labeled with heavy amino acids, mixed in equal amounts, digested with trypsin and subjected to either anti-phosphotyrosine antibody enrichment or strong cation exchange (SCX) fractionation prior to TiO₂-based phosphopeptide enrichment. The enriched phosphopeptides were then subjected to LC-MS/MS analysis.