Fig. 1.

Proteomic workflow and coverage of 20 FFPE tissue samples from DLBCL patients. A, two slices of macrodissected patient FFPE tissues were processed according to the FASP-FFPE protocol. The super-SILAC approach was used for quantitative measurements using a quadrupole Orbitrap mass spectrometer (Q Exactive). Quantification was based on SILAC ratios combined with label-free quantifications in cases where no SILAC pairs were detected. The data were analyzed using MaxQuant software, resulting in the identification of more than 9,000 proteins. B, percent coverage of signaling pathways and cellular processes in the quantified proteomes of DLBCL patients. RP, reversed phase; ECM, extracellular matrix; TCA, tricarboxylic acid.