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Detection of lipid raft specific proteins by immunoblot analysis. Flotillin-1, flotillin-2, and clathrin presented bands in all three samples tested (P- prostasomes, LR (1.5 m)- residual prostasomes in the Triton X-100 sucrose gradient, and LR- prostasomal lipid rafts). Caveolin-1 and caveolin-2 were not detected in any of the three samples.
