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. 2015 Aug 27;14(11):3040–3055. doi: 10.1074/mcp.M115.052894

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 5. — RyR2, Mfn1 and COX1 colocalize with Cx43 in the rat heart. Hearts from 10-week-old Wistar rats were maintained using a Langendorff apparatus for 10 min, followed by 20 min of perfusion (CT). Cryosections of control or ischemic hearts were immunostained using antibodies against (A) Cx43 (Sc9059) and RyR2 (upper panel), Cx43 (610062) and Mfn1 (middle panel), and Cx43 (Sc9059) and COX1 (lower panel). B, Cx43 (Sc9059) and Clathrin heavy chain (upper panel) or β-actin (lower panel). Nuclei were stained with DAPI. Scale bars, 25 μm. Arrows indicate colocalization spots. Colocalization pixel map is depicted on the right side.