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. 2015 Sep 4;14(11):3094–3104. doi: 10.1074/mcp.O115.049957

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 1. — Multiplexed Analysis. Extracted ion chromatograms for the 173 peptides (99 proteins) initially quantified by LC/MRM-MS in DBS samples. Natural (NAT) peptides are shown in blue and SIS peptides are shown in red. A total of 4 peptides were later removed during assay refinement (see Results, Final Assay): IPTTFENGR from apolipoprotein D (P05090), AASGTQNNVLR from complement component C7 (P10643), LSSGLVTAALYGR from biotinidase (P43251) and VAVNDAHLLQYNHR from galectin-3 (P17931). Because Apolipoprotein D and galectin-3 also were quantified by other peptides, the final assay consisted of 169 peptides targeting 97 proteins.