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. 2015 Oct 21;10(10):e0140991. doi: 10.1371/journal.pone.0140991

Fig 1. Immunofluorescent localization of insulator proteins in the nuclei of S2 cells overexpressing EAST or its fragments.

Fig 1

Immunofluorescent localization of insulator proteins in the nuclei of nontransfected S2 cells (control) and S2 cells overexpressing EAST, EAST1-1995, or EAST933-2362 tagged with FLAG×3. (A) Immunostaining with antibodies to Su(Hw) (green), Mod(mdg4)-67.2 (Mod-67.2, green), CP190 (red), and FLAG (blue). Scale bars, 5 μm. (B) Immunostaining with antibodies to Su(Hw) (red), Mod(mdg4)-67.2 (Mod-67.2, green), dCTCF C-terminal region (green), and FLAG (blue). Scale bars, 5 μm.