Fig 8. Role of EAST and CP190 in the expression of genes whose promoters are bound to by the insulator proteins.

(A) Changes in the expression of individual genes after CP190-specific RNAi knockdown in Drosophila S2 cells relative to that after GFP RNAi treatment (taken to be 1). The RpL32 and eh genes that showed no change in expression after the loss of CP190 were used as control. Anti-Tubulin staining (Tub) was used as loading control. The experiments were performed on two samples involving independent RNAi, RNA preparations, and RNA reverse transcription into cDNA. (B) Changes in the expression of individual genes after overexpression of EAST^1-2362, EAST^1-1995, or EAST^933-2362. The experiments were performed on three samples involving independent transfections with a full-length EAST or its fragments, RNA preparations, and RNA reverse transcription into cDNA. Error bars indicate standard deviation of two or three independent biological replicates *P ≤ 0.05 (Student’s t-test), in other cases P ≤ 0.01.