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. 2015 May 28;77(10):1253–1259. doi: 10.1292/jvms.15-0039

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©2015 The Japanese Society of Veterinary Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.

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Fig. 2. — Electrophoretic analysis of the RT-PCR product from segment 2 of EHDV isolates using IBAV-specific (A), EHDV serotype 1-specific (B) and IBAV 1997 variant-specific (C) primer pairs. Each primer set expectedly generates a specific product with the lengths 617, 919 and 370 bp, respectively. Lanes: 1, KS-1/E/13; 2, KS-2/E/13; 3, KS-5/P/13; 4, KS-7/E/13; 5, KS-8/E/13; 6, Ibaraki-2 (positive control for IBAV); 7, Kawanabe (positive control for EHDV serotype 1); 8, KSB-14/E/97 (positive control for IBAV 1997 variant); 9, negative water control; M, 100 bp DNA ladder.