Table 3. RT-PCRs and serological tests to detect the prevalence of EHDV in cattle sampled in the farms where clinical cases were observed.

Farm	Cattle	RT-PCR		Virus isolationb)		Titer of neutralizing antibody
						Ibaraki-2c)		KS-1/E/13 c)
		EHDV segment 3	IBAV segment 2	HmLu-1	BHK-21	pre	post	pre	post
A	A-1a)	+	+	–	NT	1,024	1,024	2	2
	A-2	+	–	–	NT	2	8	2	256
	A-3	+	+	–	NT	2	512	<2	<2
	A-4	–	–	–	NT	<2	16	<2	<2
	A-5	–	–	–	NT	<2	<2	<2	<2
	A-6	–	–	–	NT	<2	<2	<2	<2
B	B-1 a)	+	+	–	–	128	1,024	4	2
	B-2	–	–	–	–	<2	512	<2	2
	B-3	+	–	–	–	512	256	2	2
	B-4	–	–	–	–	<2	512	<2	2
	B-5	+	–	–	–	2	4	16	128
	B-6	+	+	–	–	64	128	<2	8
	B-7	+	+	–	+ (KS-7/E/13)	8	256	<2	<2
	B-8	–	–	–	–	<2	2	<2	<2
	B-9	+	–	–	+ (KS-8/E/13)	<2	128	<2	2

a) Cattle showing swallowing difficulty, b) Parenthesis indicates virus isolate, c) EHDV isolates used for VNTs, NT: not tested.