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Sampling from tooth surfaces using a sample collection device under constant pressure. A weight of 20 ± 5 g was loaded on the buccal surface of the left and right maxillary fourth premolars using a sample collection device under constant pressure with a sterile cotton swab (a), and the buccal surface was rubbed back and forth 3 times using the cotton swab in a parallel position to the surface to collect samples (b).
