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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Exp Eye Res. 2015 Jul 4;138:104–113. doi: 10.1016/j.exer.2015.07.001

Figure 2. Transgenic expression of rat Cryaa rescues lens phenotypes induced by α-crystallin morpholino knockdown in 4dpf embryos.

Figure 2

DIC images from wild-type (WT) lens (A), MO-cryaa lens (B), MO-cryaba lens (C) and MO-cryabb lens (D) at 4dpf demonstrating the severe lens phenotype observed upon α-crystallin knockdown. The H&E staining on histological sections from WT lens (E) and MO-cryaa lens (F). The slightly immature retina in the MO-cryaa sample might be due to non-specific effects of MOs. Western blot (G) against zebrafish Cryaa protein suggested that MO-cryaa interfered the translation of cryaa gene. RT-PCR analysis (H) of cryaba and cryabb showed that zebrafish 3B-cystallins were expressed relatively early (starting from 24hpf). Significant numbers of MO-cryabb knockdown embryos (10ng) showed heart edema and tail abnormality (I). Statistic analyses of Rno.Cryaa-rescue effect on MO-cryaa, MO-cryaba and MO-cryabb (J).