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. Author manuscript; available in PMC: 2016 Oct 15.
Published in final edited form as: Dev Biol. 2015 Sep 16;406(2):186–195. doi: 10.1016/j.ydbio.2015.08.021

Figure 2. Ephrin-B2 expression in the vascular endothelium is necessary and sufficient to allow normal CNCC migration and BA morphogenesis.

Figure 2

(A–H) Whole mount co-immunostain against the endothelial marker CD31 (A–D) and a marker of migratory neural crest Sox10 (E–H) with channels merged (I–L) in E9.5 embryos of the indicated genotypes. Embryos with endothelial rescue of ephrin-B2 (C) undergo angiogenic remodeling, hierarchical branching shown by arrows, similarly to controls (A), whereas embryos with endothelial deletion of ephrin-B2 (D) and Efnb2null embryos (B) fail to do so. Conversely, endothelial ablation of ephrin-B2 (H) and Efnb2null (F) embryos exhibit defects in neural crest migration, shown by white arrows, and dysmorphic branchial arches, shown by white arrowheads, in contrast to endothelial rescue of ephrin-B2 (G) and control (E) embryos which display normal neural crest migration and branchial arch morphogenesis. Scale bars: 200μm.