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. Author manuscript; available in PMC: 2016 Oct 15.
Published in final edited form as: Dev Biol. 2015 Sep 16;406(2):186–195. doi: 10.1016/j.ydbio.2015.08.021

Figure 4. Loss of ephrin-B2 does not affect somite polarity.

Figure 4

(A–H) Whole mount co-immunostain against neurofilament (2H3) (A–D) and ephrin-B2-GFP (E–H) in E9.5 embryos of the indicated genotypes. Efnb2null embryos (B) and embryos with endothelial deletion of Efnb2 (D) fail to initiate DRG morphogenesis, in contrast to embryos with endothelial rescue of Efnb2 (C) and controls (A). (I–J) Whole mount in situ hybridization for Sema3F on E9.5 embryos of the indicated genotypes. Asterisks indicate the forelimb bud position. Scale bars: 200μm.