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. Author manuscript; available in PMC: 2016 Oct 15.
Published in final edited form as: Dev Biol. 2015 Sep 16;406(2):186–195. doi: 10.1016/j.ydbio.2015.08.021

Figure 5. Elevated apoptosis in the CNCC in the absence of vascular endothelial ephrin-B2.

Figure 5

(A–D′) Immunostaining for cleaved Caspase3 and Sox10 on cryosections of E9.75 embryos of indicated genotypes and high magnification insets. Efnb2null embryos (B) and Efnb2GFP/lox; Tie2-CreTg/+ embryos with endothelial deletion of ephrin-B2 (D) exhibit large amounts of cell death localized to the migratory and post-migratory CNCCs compared with control (E) or Efnb2CR/GFP; Tie2-CreTg/+ embryos with expression of ephrin-B2 only within the VE (G). In the trunk region elevated apoptosis is also associated with, but not limited to migratory NCCs in Efnb2null embryos (J,N,R), or Efnb2GFP/lox; Tie2-CreTg/+with deletion of ephrin-B2 from the VE (L,P,T) compared to control (A) or Efnb2CR/GFP; Tie2-CreTg/+embryos with expression of ephrin-B2 only within the VE (G,K,O,S). Asterisks indicate forelimb bud. Scale bars: A–D 100μm, A′–D′ 25μm, E–L 200μm, M–T 100μm.