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. 2015 Nov 10;6:172. doi: 10.3389/fendo.2015.00172

Figure 2.

Figure 2

The effect of post-weaning social isolation on body weight and reproductive neuropeptide gene expression in the brain. (A) Body weight changes during post-weaning social isolation (n = 6/housing). (B) Post-weaning social isolation decreased the levels of gonadotropin-releasing hormone mRNA in the pre-optic area (n = 6/housing) but did not change the levels of gonadotropin-inhibitory hormone mRNA in the hypothalamus (n = 6/housing). The relative mRNA expression levels were normalized to that of inosine 5-monophosphate dehydrogenase 2 mRNA. All data are presented as mean ± SEM. Significant differences were determined using the Student’s t-test for unpaired values; significance was set at *p < 0.05.