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. 2015 Nov 10;6:172. doi: 10.3389/fendo.2015.00172

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Copyright © 2015 Soga, Teo, Cham, Idris and Parhar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of post-weaning social isolation on enhanced green fluorescent protein–gonadotropin-inhibitory hormone neurons in the dorsomedial hypothalamic nucleus. (A) Total number of gonadotropin-inhibitory hormone (GnIH) cells in the dorsomedial hypothalamic nucleus (DMN) of control and isolated male rats (control = 14 and isolated = 11). (B) Percentage of c-Fos-positive GnIH neurons in the DMN of control and isolated male rats. Data represent the mean ± SEM for each group. *p < 0.05. (C) Confocal images of enhanced green fluorescent protein (EGFP)–GnIH cells expressing c-Fos protein (white, indicated by arrows), EGFP–GnIH neurons (green), and red c-Fos protein (magenta) in control (left panel) and isolated (right panel) male rats. Scale bar: 100 μm.