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. 2015 Nov 10;6:172. doi: 10.3389/fendo.2015.00172

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Copyright © 2015 Soga, Teo, Cham, Idris and Parhar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Co-localization of 5-hydroxytryptamine fibers and the 5-hydroxytryptamine_2A receptor in enhanced green fluorescent protein–gonadotropin-inhibitory hormone cells in the dorsomedial hypothalamic nucleus. (A) Enhanced green fluorescent protein (EGFP)–gonadotropin-inhibitory hormone (GnIH) cells and fibers (green), (B) 5-hydroxytryptamine _2A (5-HT_2A)-immunostained neurons (red), and (C) EGFP–GnIH cells expressing 5-HT_2A (yellow/orange) and 5-HT_2A-immunostained neurons (red) in the dorsomedial hypothalamic nucleus (DMN). (D) EGFP–GnIH neurons (green), (E) 5-hydroxytryptamine (5-HT)-immunostained fibers (red), and (F) 5-HT in close juxtapositions in EGFP–GnIH neurons or fibers (yellow dot, indicated by arrows). (G) 3D images of 5-HT in close juxtapositions in EGFP–GnIH neurons or fibers (yellow dot, indicated by arrows) in the DMN. Scale bars: (A–C), 50 μm; (D–G), 20 μm.