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. 2015 Nov 10;5:16478. doi: 10.1038/srep16478

Figure 2. DHTS inhibits HuR’s first RRMs and is not effective against other RBPs, HuD excluded; in vitro activity of other tanshinones.

Figure 2

(A) Representative gels of at least three independent protein preparations of recombinant M1_M2 and M2_M3 HuR proteins. REMSAs were performed with 0.5 μM of protein, 0.5 μM of Cy-3 RNA probe and DMSO or DHTS at indicated doses. (B) Quantification of specific HuR-RNA binding challenged by indicated concentrations of commercially available tanshinones, including cryptotanshinone; TanI and TanII that were not included in the first screening. (C) Evaluation of DHTS activity at the indicated concentrations against Lin28b, TTP, HuD, and TDP-43 RNA-binding proteins tested by REMSA with 0.5 μM of Cy-3 RNA as indicated in methods. Mean ± SD refers to three independent experiments (n = 3).