Figure 6. Effect of DHTS on HuR sub-cellular localization.
(A) Representative immunofluorescence showing nuclei (Hoechst) or endogenous HuR (green) in MCF-7 cells treated with 1 μM DHTS or 2 μM of Actinomycin for 3 hD. (B) Representative western blot of total protein levels of HuR. (C) Western blot analysis on nuclear and cytoplasmic extracts of MCF-7 treated as in A. Densitometric analysis plot data of three independent experiments (n = 3).