Skip to main content
. 2015 Nov 10;5:16466. doi: 10.1038/srep16466

Figure 4. Stimulation of infra-patellar fat pad derived fibroblasts with IL-1α and IL-1β increases secretion of inflammatory mediators.

Figure 4

Fibroblasts isolated from infra-patellar fat pad (n = 5) were stimulated with increasing doses of LPS (0.1–20 μg/ml), HMGB1 (1–200 ng/ml), PolyIC (0.1–20 μg/ml), IL-1α and IL-1β (both 25–1000 pg/ml) for 24 hours and the secreted levels of GM-CSF (a), IL-6 (b) and IL-8 (c) determined by ELISA. All three molecules demonstrated a dose dependant increase in secretion in response to PolyIC, IL-1α and IL-1β. LPS induced maximum release of all three molecules at the lowest concentration used (0.1 μg/ml) with higher concentrations having no additional effect. The cells showed no significant response to HMGB-1. Data is presented as mean ± standard error of the mean with statistical significance compared to control indicated by; *p < 0.05, **p < 0.01 and ***p < 0.001.