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. 2015 Nov 10;5:16426. doi: 10.1038/srep16426

Figure 1. ADAM9 activates CDCP1 protein expression and enhances cell migration.

Figure 1

(A) Western blot of ADAM9 and CDCP1 in Bm7-shGFP (control) and -shADAM9 cells. ADAM9 proteins were detected in non-reducing gels using the antibody from R&D (MAB939, R&D Systems). CDCP1 proteins contain full-length and proteolytically processed forms. EF1α was used as protein loading control. (B) Immunohistochemical analysis of CDCP1 in ADAM9-depleted Bm7 cells. Scale bar: 20 μm. (C) Migration ability of Bm7-shGFP, -shADAM9, and -shCDCP1 cells using time-lapse video microscopy. Top: representative graph of motile activity of 15 randomly chosen cells. Migration traces to the right are shown in red, and to the left in black. Bottom: quantitation of migration distance over 16 h from three independent experiments. (D) Western blots of F4 cells overexpressing EGFP (F4-EGFP) and HA-CDCP1 (F4-HA-CDCP1) by retrovirus infection. (E) Migration ability of individual F4 cell overexpressing EGFP or CDCP1 using time-lapse video microscopy (top). The accumulated migration distance for 16 hours was quantified (bottom). Error bars, SD; **P < 0.01.