Table 2. Comparison of existing assay approaches and parameters with the AO-GMT (confocal microscopy-based, HTS) and GFP-GMT (light microscopy-based, proof of principle) assays.

Assay	AO-GMT assay	GFP-GMT assay	Female assay14	Dual M/F assay13	Female assay20
reagent for readout	acridine orange	GFP	conjugated Mabs	conjugated Mabs	conjugated Mabs
time after compound incubation	2.5 h	10 min	16 h	20 min + 24 h	24 h
plate format	384 wells	384 wells	384 wells	96 wells	384 wells
gametocytes/well	4 × 104	4 × 104	2 × 105	NA	8 × 103
readout(s)	gametocytes and female gametes	spherical gametes	female gametes	male and female gametes	female gametes
Z’	0.69 (gametes) 0.66 (gametocytes)	0.76	0.72	0.43 (male) 0.36 (female)	0.7