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. 2015 Nov 10;5:16435. doi: 10.1038/srep16435

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Schematic representation of the circular RNAs used in this study. The grey dashed line represents the point of ligation of the linear RNA to form a circle. (B) Nucleotide sequence of 4× FLAG circular RNA. The first nucleotide, G, is linked with the last nucleotide, U, in this context. Sequences of the other RNAs are shown in Supplementary Table S1. (C) Western blot analysis of the translation reaction in RRL. The three circular RNAs and their linear precursors (1 μg) were incubated at 30 °C overnight in a 25 μL reaction.