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. 2015 Nov 10;5:16300. doi: 10.1038/srep16300

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Competitive affinity chromatography column with NAP bound to SulfoLink® coupling resin loaded with recombinant EB3 with or without RIS. Samples were separated by electrophoresis on SDS polyacrylamide gel (12%) followed by staining with Coomassie Brilliant Blue (CBB). The stained gel results are shown. (A) Binding to NAP (control column): recombinant EB3 (3 mg) was loaded, EB3 presence in the following fractions: load, flow- through and elution (number 2–5), validates EB3-NAP interaction, with EB3 eluting with high acid concentrations (lanes 3–5). (B) RIS (3 mg) and EB3 (3 mg) were loaded together, EB3 presence in the following fractions: flow- through and wash (number 1) and not in the elution fractions indicates that RIS prevented EB3-NAP interaction.