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. 2015 Nov 10;5:16300. doi: 10.1038/srep16300

Figure 3. Doxycycline inhibits transgene synthesis.

Figure 3

Western blot results for DISC1 protein in DD-treated Tg mice vs. DD-treated WT mice in the prefrontal cortex and the hippocampus area. The band that represents the normal DISC1 protein has a molecular weight of ~110 kDa and the band underneath it represents the truncated human mutant DISC1 with a molecular weight of ~70k Da. The expression of DISC1 protein in mice without DOX treatment in the cortex area (A) and the hippocampus area (C) is presented. Only the truncated mutant DISC1 band is presented in the Tg mice group, this verifies the difference between the Tg mice, which express the human mutant protein and the WT mice which express only the normal DISC1 protein. The expression of DISC1 protein in mice with DOX treatment in the cerebral cortex area (B) and the hippocampus area (D) is presented. In figures B and D, only the normal DISC1 band is presented, which confirms that DOX treatment blocked the expression of the human mutant DISC1 protein.