Skip to main content
. 2015 Nov 10;5:16386. doi: 10.1038/srep16386

Figure 2. Facilitatory role of RESV on MET is dependent on SIRT1.

Figure 2

After transfection with control siRNA, SIRT1 siRNA, empty vector, and SIRT1-encoding vector, RPE cells were incubated for 48 h. (A) mRNA expression of SIRT1, E-cadherin and α-SMA are shown as relative fold to control siRNA normalized to GAPDH. **P < 0.01. *P < 0.05. Data are presented as mean ± SEM. n = 4/group. (B) Western blot analysis of SIRT1, E-cadherin, α-SMA and GAPDH in the cell lysates of RPE cells. An expanded image of the western blot of E-cadherin is shown in Supplementary Figure 6. (C) After transfection with control siRNA and SIRT1 siRNA, the cells were incubated with RESV at 50 μM for 48 h. mRNA expression of SIRT1, E-cadherin and α-SMA are shown as relative fold to control siRNA normalized to GAPDH. **P < 0.01. *P < 0.05. Data are presented as mean ± SEM. n = 4/group. (D) Western blot analysis of SIRT1, E-cadherin, α-SMA and GAPDH in the cell lysates of RPE cells. An expanded image of the western blot of E-cadherin is shown in Supplementary Figure 6.