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. 2015 Nov 9;211(3):569–586. doi: 10.1083/jcb.201506048

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© 2015 Schreiber et al.

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Figure 9. — Fear memory consolidation is increased in Actg1 cKO mice. (A) Actg1 was conditionally deleted in forebrain regions by crossing Actg1^lox/lox mice. Immunoblotting of total protein lysates confirmed a reduction of ACTG1 protein levels and a compensatory upregulation of ACTB levels in hippocampus, frontal cortex, and olfactory bulb of Actg1 cKO mice. No changes were observed in the levels of TUBB3, GAPDH, or TRIM3, and no reduction in ACTG1 protein levels was observed in brainstem or cerebellum. (B) ACTG1 protein levels in forebrain areas (hippocampus, frontal cortex, and olfactory bulb) of Actg1 cKO mice were reduced 70–90% relative to wild-type control tissue, whereas ACTB levels were 60% increased (means ± SEM, two-tailed t test, **, P < 0.01). (C) Hippocampal memory performance was tested at 3 mo of age in a contextual fear memory task. Freezing behavior was significantly increased in Actg1 cKO mice compared with wild-type littermates when reexposed to the conditioned context 24 h or 72 h after receiving the shock (means ± SEM, two-tailed t test, *, P < 0.05, n = 5/6 per genotype).