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. 2015 Nov 9;211(3):639–652. doi: 10.1083/jcb.201505062

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© 2015 Li et al.

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Figure 2. — ESCRTs, but not the autophagy machinery, are required for the degradation of Cot1-GFP. (A) Localization of tet-O7 Cot1-GFP in WT and autophagy mutants 4 h after Zn²⁺ withdrawal from YNB media and addition of 2 µg/ml Dox. (B) Degradation kinetics for tet-O7 Cot1-GFP in WT and autophagy mutants. G6PDH was used as a loading control. Same volume of cells was loaded, with 1 OD₆₀₀ cells loaded at 0 h. (C) Localization of tet-O7 Cot1-GFP 4 h after Zn²⁺ withdrawal in WT and ESCRT mutants. Arrows highlight the aberrant endosomes. (D) Degradation kinetics for tet-O7 Cot1-GFP in WT and ESCRT mutants. G6PDH was used as a loading control. Same volume of cells was loaded, with 1 OD₆₀₀ cells loaded at 0 h. Bar, 1 µm.