Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Nov 9;211(3):639–652. doi: 10.1083/jcb.201505062

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Li et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 3. — Tul1, but not the VAcUL-1 complex, is required for Cot1-GFP degradation. (A) ssh4Δ and rsp5-1 mutants did not block the internalization of Cot1-GFP into the vacuole lumen after Zn²⁺ withdrawal from the YNB media. (B) Degradation kinetics for tet-O7 Cot1-GFP in WT, ssh4Δ and rsp5-1 mutants. G6PDH was used as a loading control. Same volume of cells was loaded, with 1 OD₆₀₀ cells loaded at 0 h. (C) The internalization of Cot1-GFP into the vacuole lumen was blocked in tul1Δ strain after Zn²⁺ withdrawal from the YNB media. (D) Degradation kinetics for tet-O7 Cot1-GFP in WT and ssh4Δ strains. G6PDH was used as a loading control. Same volume of cells was loaded, with 1 OD₆₀₀ cells loaded at 0 h. Bar, 1 µm.